Table 2.
Effects of each QTL allele detected for single seed weight (SSW) on other seed yield components in the NsT-RILs grown at Mito and Tsukuba experimental conditions
| QTL | Allele | Experimental condition | SSW (mg) | NP | NOP | RSS (%) | SY (g) |
|---|---|---|---|---|---|---|---|
| qSw12-1 (Sat_206) | ‘Natto-shoryu’ (n = 90) | Mito | 216 ± 36 | 155 ± 39 | 2.27 ± 0.17 | 71.6 ± 8.0 | 53.6 ± 15.8 |
| Tsukuba | 220 ± 30 | 165 ± 32 | 2.31 ± 0.19 | 85.2 ± 4.7 | 70.2 ± 12.0 | ||
|
| |||||||
| ‘Tachinagaha’ (n = 74) | Mito | 244 ± 31 | 130 ± 28 | 2.29 ± 0.19 | 67.8 ± 10.1 | 48.7 ± 12.2 | |
| Tsukuba | 242 ± 29 | 139 ± 28 | 2.32 ± 0.20 | 83.5 ± 5.3 | 63.7 ± 11.3 | ||
|
| |||||||
| ANOVA | Allele | *** (0.130) | *** (0.132) | NS (0.003) | *** (0.017) | *** (0.034) | |
| Experimental condition | NS (0.000) | ** (0.018) | NS (0.009) | *** (0.492) | *** (0.261) | ||
| Interaction | NS (0.002) | NS (0.000) | NS (0.000) | NS (0.002) | NS (0.001) | ||
|
| |||||||
| qSw13-1 (CSSR535) | ‘Natto-shoryu’ (n = 89) | Mito | 219 ± 39 | 143 ± 37 | 2.38 ± 0.14 | 68.7 ± 9.4 | 50.3 ± 14.1 |
| Tsukuba | 218 ± 31 | 151 ± 35 | 2.42 ± 0.17 | 85.1 ± 5.2 | 65.9 ± 12.9 | ||
|
| |||||||
| ‘Tachinagaha’ (n = 78) | Mito | 238 ± 32 | 144 ± 36 | 2.17 ± 0.14 | 70.2 ± 9.7 | 51.7 ± 15.4 | |
| Tsukuba | 241 ± 28 | 157 ± 31 | 2.21 ± 0.15 | 84.3 ± 5.1 | 68.9 ± 11.5 | ||
|
| |||||||
| ANOVA | Allele | *** (0.093) | NS (0.002) | *** (0.321) | NS (0.000) | NS (0.005) | |
| Experimental condition | NS (0.000) | * (0.020) | * (0.010) | *** (0.499) | *** (0.266) | ||
| Interaction | NS (0.001) | NS (0.002) | NS (0.000) | NS (0.003) | NS (0.001) | ||
|
| |||||||
| qSw17-1 (CSSR172) | ‘Natto-shoryu’ (n = 96) | Mito | 222 ± 36 | 146 ± 38 | 2.26 ± 0.18 | 69.7 ± 9.9 | 50.1 ± 14.2 |
| Tsukuba | 223 ± 30 | 156 ± 34 | 2.29 ± 0.18 | 85.3 ± 4.7 | 66.5 ± 12.5 | ||
|
| |||||||
| ‘Tachinagaha’ (n = 70) | Mito | 240 ± 35 | 132 ± 29 | 2.27 ± 0.17 | 68.5 ± 9.0 | 48.6 ± 12.1 | |
| Tsukuba | 239 ± 32 | 148 ± 29 | 2.31 ± 0.20 | 84.1 ± 5.4 | 67.1 ± 11.1 | ||
|
| |||||||
| ANOVA | Allele | *** (0.062) | ** (0.024) | NS (0.001) | NS (0.003) | NS (0.000) | |
| Experimental condition | NS (0.000) | *** (0.032) | NS (0.009) | *** (0.510) | *** (0.317) | ||
| Interaction | NS (0.000) | NS (0.002) | NS (0.000) | NS (0.000) | NS (0.001) | ||
|
| |||||||
| qSw20-1 (Ln) | ‘Natto-shoryu’ (n = 79) | Mito | 240 ± 34 | 139 ± 33 | 2.16 ± 0.13 | 68.9 ± 9.6 | 49.0 ± 13.2 |
| Tsukuba | 241 ± 28 | 159 ± 33 | 2.17 ± 0.12 | 85.1 ± 5.0 | 69.5 ± 12.1 | ||
|
| |||||||
| ‘Tachinagaha’ (n = 91) | Mito | 218 ± 35 | 147 ± 39 | 2.39 ± 0.14 | 69.9 ± 9.2 | 52.8 ± 15.7 | |
| Tsukuba | 218 ± 31 | 147 ± 32 | 2.45 ± 0.15 | 84.5 ± 4.9 | 65.0 ± 11.8 | ||
|
| |||||||
| ANOVA | Allele | *** (0.109) | NS (0.001) | *** (0.465) | NS (0.000) | NS (0.000) | |
| Experimental condition | NS (0.000) | * (0.018) | * (0.009) | *** (0.511) | *** (0.262) | ||
| Interaction | NS (0.000) | ** (0.020) | NS (0.003) | NS (0.001) | ** (0.017) | ||
The genotype at each environmentally stable QTL for SSW was estimated by the alleles of the flanking markers; Sat_206 for qSw12-1, CSSR535 for qSw13-1, CSSR172 for qSw17-1, and Ln for qSw20-1.
SSW, single seed weight; NP, number of pods per plant; NOP, number of ovules per pod; RSS, rate of seed-set; SY, seed yield per plant.
Trait value of each genotype group is shown as mean ± standard deviation.
represent statistical significance at P < 0.05, 0.01, and 0.001 by ANOVA, respectively; NS = not significant.
Values in parentheses indicate effect size (η2) of each factor calculated as a proportion of the sum of squares of the factor to the total sum of squares.