Fig. 4. BPC comparison of (a) Pyxidicoccus fallax An d48 wild type extract, the extracts of the (b) pcyC and (c) pcyE gene inactivation mutants, and anthraquinones produced in P. fallax An d48 (d) vanillate-induced and (e) tn5 activation mutants. The three anthraquinone metabolites 340 (3), 296 (5) and 325 (4) are displayed as LC-MS EICs at 341.06 [M + H]⁺ (light green), 325.07 [M + H]⁺ (dark blue) and 297.075 [M + H]⁺ (orange). The corresponding structure formula is shown in the same colour. Differences visible in traces (a) to (e) at retention times later than 12 minutes are unrelated to the investigated biosynthetic route.