Fig 3.

Sequence of steps in the preparation of the BioCartilage graft, beginning with preparation of autologous conditioned leukocyte-reduced platelet-rich plasma (PRP) using a sample of the patient's blood and the PRP system of choice (A). A peripheral blood draw by the anesthesia team should be obtained before incision to optimize the biological milieu of the PRP. (B) The dehydrated scaffold (bottom) is mixed with the PRP (top) in a 1:0.8 scaffold–PRP volume ratio within the mixing syringe provided with the scaffold. A dryer mix is preferable because over-hydration may impair handling and forming properties and may lead to graft washout. (C) The arthroscopic delivery needle is loaded with the prepared BioCartilage graft from the mixing syringe, and an obturator is inserted to facilitate graft delivery to the lesion.