Table 1.

Primer sequences used in the study for mRNA quantification with RT-PCR in rat ACLFs.

Gene	Sequence	Annealing temperature (°C)	Refs.
Collagen type I	5′-TCA CCT ACA GCA CGC TTG-3′ 5′-GGT CTG TTT CCA GGG TTG-3′	55	[45]
Collagen type III	5′-ATA TCA AAC ACG CAA GGC-3′ 5′-GAT TAA AGC AAG AGG AAC AC-3′	52	[45]
TGF-β1	5′-TGC TAA TGG TGG ACC G-3′ 5′-ACG TCA AAA GAC AGC C-3′	52	[46]
GAPDH	5′-ACA TCA TCC CTG CAT CCA CT-3′ 5′-GGG AGT TGC TGT TGA AGT CA-3′	55	[45]

ACLFs = anterior cruciate ligament fibroblasts; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; RT-PCR = reverse transcription-polymerase chain reaction; TGF-β1 = transforming growth factor-β1.