Fig. 6.

Time course of aggregation reaction monitored with 7MFE. a apparent contributions in fractional concentration units (x1, x3) to spectral signal from sp1 (monomer spectrum, blue), and sp3 (fibril spectrum, orange); green: sum; light scatter (inset) midpoint at ~ 33 h. b relative contributions to spectral signal by sp1 (blue), sp2 (intermediate with 0.2 relative quantum yield, orange), and sp3 (green); c normalized contributions (in concentration units) to spectral signal by sp1 (blue), sp2 (orange), and sp3 (green); the sum is = 1. d normalized contributions to fluorescence signal at 540 nm by sp1 (blue), sp2 (orange), and sp3 (green); time course of signal at 540 nm (F540, red)