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Validation of transgene expression in PC3s. a, b PC3s were transfected with RALA/pEGFP-N1 (comprising 0.5 μg DNA) at N:P10 for 6 h. Cells were analyzed for GFP expression 48-h post-transfection using immunoblotting, and fluorescence microscopy. c, d PC3s were transfected with RALA/CMV-iNOS (comprising 0.5 μg DNA) at N:P10 for 6 h. Cells were analyzed for iNOS expression 48-h post-transfection using immunoblotting, for ·NO generation by Greiss test. Datapoints represent mean ± SD; N = 3
