Changes in the activation free energy (A), enthalpy (B), and entropic component (C) in cosolutes. Folding, black; unfolding, red. Changes in the activation free energy were determined at 303 K. Changes in activation enthalpy and entropy were determined at 303 K for folding, and without a reference temperature for unfolding, as described in the text. Uncertainties (one standard deviation) in panel A are smaller than the points. Changes in entropy were multiplied by 303 K. All values and uncertainties are listed in Table S5.