Table 1.
Identified anti-nitrotyrosine immunopositive proteins in livers of LPS-treated rats from Fig. 4
| Protein | Coverage | Peptide match | Function | Location | |
|---|---|---|---|---|---|
| 1 | Voltage-dependent anion channel-1 | 49% | 9 | Mitochondrial anion channel. Part of PTP. Involved in apoptosis | Mit |
| 2 | Mitochondrial aconitase | 24% | 13 | Metabolic enzyme—Citrate to isocitrate (citric acid cycle) (iron) | Mit |
| 3 | ATP synthase α-chain F1 ATPase | 14% | 5 | ATP production | Mit |
| 4 | Glutathione S-transferase (μ type) | 43% | 9 | Detoxification, steroid-selective, and oxidative stress | Cyto |
| 5 | Catalase | 43% | 18 | Breakdown of hydrogen peroxide and oxidative stress (iron) | Cyto |
| 6 | MnSOD | 19% | 4 | Breakdown of superoxide and oxidative stress (manganese) | Mit |
| 7 | Carbonic anhydrase III | 53% | 11 | Hydratation of CO2. Protection to oxidative damage (zinc) | Cyto |
| 8 | hnRNP A2/B1 | 34% | 7 | Vitamin D-binding protein? An early indicator of some cancers | Cyto |
| 9 | RACK-1 guanine nucleotide-binding protein | 28% | 7 | Activated protein kinase C receptor | Cyto |
| 10 | Annexin IV | 20% | 6 | Involved in exocytosis-binds Ca2+/phospholipid (calcium) | Cyto |
| 11 | Purine nucleoside phosphorylase | 22% | 5 | Nucleoside metabolism-implicated in apoptosis | Cyto |
| 12 | β-Tubulin chain 15 | 21% | 8 | Structural protein | Cyto |
| 13 | Sensescene marker protein 30 (Regucalcin) | 37% | 10 | Calcium-binding protein. Sensescene marker. (calcium) | Cyto |
| 14 | Aldolase B-fructose bisphosphatase | 32% | 11 | Fructose metabolism: glycolysis | Cyto |
| 15 | Mit. glutamate oxaloacetate transaminase-2 | 28% | 11 | Aspartate aminotransferase transaminase family | Mit |
| 16 | Transketolase | 15% | 5 | Involved in glycolysis | Cyto |
| 17 | Hydroxymethylglutaryl-CoA synthase | 18% | 10 | glutaryl-CoA synthesis-ketogenesis | Mit |
| 18 | 4-trimethylamino butyrataldehyde dehydrogenase | 26% | 9 | Carnitine synthesis (NAD+) | Mit |
| 19 | Glycine methyl transferase | 21% | 5 | Methyltransferase | Cyto |
| 20 | Glyceraldehyde phosphate dehydrogenase | 18% | 5 | Key enzyme in glycolysis (NAD+) | Cyto |
| 21 | 3-α-OH steroid dehydrogenase | 50% | 13 | Quinone reduction, aromatic alcohol and 15-OH prostaglandin dehydrogenase. Inhibited by NSAID (NADP+) | Cyto |
| 22 | Glutamate dehydrogenase | 21% | 6 | Citric acid cycle—malate to oxaloacetate (NAD(P)+) | Mit |
| 23 | 3-oxo-5 β steroid dehydrogenase | 16% | 5 | Bile acid and steroid hormone metabolism (NADP+) | Cyto |
| 24 | Short chain 3-OH acyl CoA dehydrogenase | 28% | 5 | Mitochondrial β-oxidation of short chain fatty acids (NAD+) | Mit |
| 25 | D-β-OH butyrate dehydrogenase | 15% | 4 | β-oxidation of fatty acids (NAD+) | Mit |
| 26 | 3-ketoacyl CoA thiolase | 36% | 15 | Involved in β oxidation of fatty acids | Mit |
| 27 | Lamin A | 15% | 5 | Structural protein | Nuc |
| 28 | Homogenisate 1,2 dioxygenase | 18% | 7 | Key enzyme in alkaptonuria in mice | Cyto |
| 29 | Betaine homocysteine S-transferase | 32% | 9 | Regulation of homocysteine metabolism (zinc) | Cyto |
| 30 | 3 Mercaptopyruvate sulfur transferase | 29% | 7 | Cyanide degradation and thiosulfate biosynthesis | Cyto |
| 31 | Rhodanese (thiosulfate sulfurtransferase) | 25% | 10 | Mitochondrial thiosulfate sulfurtransferase | Mit |
Identified nitrated protein in the liver of LPS-treated rats are shown with the number of peptide that matched the protein and percentage coverage of the protein. Cofactors required for activity are indicated in bold. Location of the protein is also indicated: mit, mitochondrial; Cyto, cytoplasmic; Nuc, nuclear.