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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Gene Rep. 2017 Dec 28;10:149–156. doi: 10.1016/j.genrep.2017.12.004

Figure 3.

Figure 3

Expression of tendon/ligament related extracellular matrix genes in MSCs by RT-qPCR. Cells belonging to 4 different treatment groups (Control = PL alone; FGF2, GDF5, FGF2 + GDF5) over 4 time points (Day 1, Day 3, Day 7 and Day 14) were plated and RNA isolated and processed as described in methods. X-axis indicates days of culture, with day 1 as actively dividing MSCs. Gene expression normalized to GAPDH, n=2. (A) Expression of Extracellular Matrix Genes. COL1A1, collagen type I; COL3A1, collagen type III; TNC, Tenascin-C; TNMD, Tenomodulin; (B) Expression of Proteoglycan Genes. Decorin, DCN; Aggrecan, ACAN; Scleraxis, SCX; (C) Expression of Nuclear Lamina Genes. Lamin A, LMNA; Lamin B1, LMNB1; Lamin B2, LMNB2.