Table 2. List of primers for pluripotency and differentiation markers.

Target genes	Primer sequence
OCT4
Forward	CCTCACTTCACTGCACTGTA
Reverse	CAGGTTTTCTTTCCCTAGCT
SOX2
Forward	CCCAGCAGACTTCACATGT
Reverse	CCTCCCATTTCCCTCGTTTT
NANOG
Forward	AAGGTCCCGGTCAAGAAACAG
Reverse	CTTCTGCGTCACACCATTGC
Actin
Forward	CCCAGAGCAAGAGAGG
Reverse	GTCCAGACGCAGGATG
FOXA2
Forward	CCAACCCCACAAAATGGA
Reverse	ATAATGGGCCGGGAGTACA
SOX17
Forward	ACCGCACGGAATTTGAAC
Reverse	GCAGTAATATACCGCGGAGC
BRACHYURY
Forward	CCCTATGCTCATCGGAACA
Reverse	TTCCAAGGCTGGACCAAT
MSX1
Forward	TCCGCAAACACAAGACGA
Reverse	ACTGCTTCTGGCGGAACTT
MAP2
Forward	TGAAGCAAAGGCACCTCAC
Reverse	TATGGGAATCCATTGGCG
PAX6
Forward	TTGCTTGGGAAATCCGAG
Reverse	TGCCCGTTCAACATCCTT
GAPDH
Forward	GGTGGTCTCCTCTGACTTC
Reverse	CTCTTCCTCTTGTGCTCTTG