Figure 3. Microarray analysis of ALDH^highCD44^high and ALDH^lowCD44^low cells from control and cisplatin-treated UM-SCC-22B.

(A) Histogram plot of log2 intensity values for 16 microarrays following batch adjustment. (B) Principal component analysis of batch adjusted 16 microarrays showing the 1st and 2nd components with the most variance. Grey circles–Control ALDH^highCD44^high; yellow squares–Control ALDH^lowCD44^low; black diamonds–Cisplatin ALDH^highCD44^high; blue triangles–Cisplatin ALDH^lowCD44^low. Exp #1: 1–4; Exp #2: 5–8; Exp #3: 9–12; Pilot Exp: 13–16. (C) Unsupervised hierarchical clustering with complete linkage and Euclidean distance was performed on only statistically significant probes (adjusted p-value < 0.05).