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. 2018 May 18;9(38):25193–25205. doi: 10.18632/oncotarget.25391

Figure 3.

Figure 3

(A) Impact of high salt treatment on paclitaxel resistance. MCF-7 cells were cultured under basal conditions; MCF-7s cells were passaged eight times in high salt added cell culture media (Δ0.05 M NaCl). (B) Pharmacokinetic parameters of paclitaxel cytotoxicity following various treatment conditions. (C) Western blot analysis of P-glycoprotein expression following high salt treatment. MCF-7s, represent MCF-7 cells were passaged eight times in high salt added cell culture media (Δ0.05 M NaCl); 231s, represents MDA-MB-231 cells passaged eight times in high salt added cell culture media (Δ0.05 M NaCl). (D) Impact of high salt treatment on intracellular Rhodamine-123 accumulation. Rhodamine 123 is pumped out of the cell during enhanced expression of P-glycoprotein. (E–G) The high salt treated cells (for 8 passages) were latter cultured in normal salt containing basal media for 5 passages (referred to as MCF-sr) and then tested for the expression of Orai1 (E), STIM1 (F) and P-glycoprotein (G). Data were representative of five experiments and shown as mean ± SEM, p < 0.05.