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. 2018 May 18;9(38):24950–24969. doi: 10.18632/oncotarget.25116

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Copyright: © 2018 Karthikeyan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Histogram depicts MTS assay absorbance values at 24, 48 and 72h after treatment of glioma cells with conditioned medium derived from microglia. A significant increase in glioma viability was observed after treatment with microglia conditioned medium at all time points (A). This was further confirmed using alamar blue viability assay wherein an increase in % of reduction of alamar blue was observed in glioma cells treated with microglia conditioned medium at all time points (B). Histogram further shows the MTS assay absorbance values at 24, 48 and 72h treatment of glioma cells with conditioned medium derived from shSMAD4 microglial cells (C). A decrease in absorbance values indicates a decrease in viability of glioma cells after treatment with conditioned medium from shSMAD4 cells as compared to glioma cells treated with negative control medium (C). In addition, alamar blue assay results (D) show a decrease in percentage of alamar blue reduction in glioma cells treated with medium from shSMAD4 microglia as compared to glioma cells treated with negative control medium. Data represent Mean±SD, (n=4), ^***p<0.001, ^****p<0.0001.