Figure 3.
Growth of yeast strains containing integrated copies of tra1 with mutations of R3389, R3390 and/or R3456 to glutamine. (A) TRA1 (CY4353), tra1-R3456Q (CY6554), tra1-R3389Q, R3390Q (CY6517) and tra1-R3389Q, R3390Q, R3456Q (tra1; CY6582) were grown to saturation in media lacking leucine and spotted in 10-fold serial dilutions onto a YPD plate at 30° or onto a YPD plate containing 6% ethanol or 6 μg/ml Calcoflour white or onto a YPD plate at 37°. Strains were incubated for 3-4 days. (B) TRA1 (CY4353) and tra1 (CY6582) were grown to saturation in media lacking leucine then spotted in 10-fold serial dilutions onto YPD media or YPD media depleted of phosphate, YPD media containing MMS or caffeine, minimal media lacking inositol, minimal media containing calcium or salt or a YP plate containing 2% galactose. Strains were incubated for 2-5 days at 30°.