Figure 5.
tra1 affects expression from SAGA regulated promoters. TRA1 (BY4741) and tra1 (CY6635) were transformed with the indicated promoter-lacZ fusion molecules on a LEU2 centromeric plasmid. Strains were grown to saturation in media lacking leucine before being diluted 1:10 in YPD with 6% ethanol for the SRE reporter, media lacking phosphate for the PHO5 promoter, YP with galactose for the GAL10 promoter or YPD for the ARG1 and NCW2 promoters and grown for eight hours. β-galactosidase activity is shown as the average of six replicates for the regulated promoters and three replicates for the NCW2 promoter with the SD indicated by the error bars.