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tti2-F328 is synthetically lethal with tra1. Both TRA1 (CY4353) and tra1 (CY6582) were transformed with either YCplac33 (-), YCplac33-TTI2 (WT) or YCplac33-tti2-F328S (F328S). Strains were grown to saturation in media lacking leucine and uracil, plated in 10-fold serial dilutions on YPD and grown for two days at 30°.
