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. 2018 Apr 16;8(6):1993–2006. doi: 10.1534/g3.118.200229

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Copyright © 2018 Dannah et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of hif1Δ hat2Δ double knockout cells for growth defects on YPD media. A: Strains were grown to an OD at 600nm of ≅0.5 before being plated at fivefold serial dilutions on YPD. B: Strains were cloned into pRB4151-2MYC lacking the Leucine amino acid (-Leu) for selectivity. Hif1 F.L. was transformed into hif1Δ/hat2Δ and hif1Δ to rescue the phenotype. C, D: Various Hif1 truncations were expressed back into hif1Δ hat2Δ double knockout cells to examine their ability to rescue the phenotype. Note: C1 and D1 represent 3 days of growth. E: Western blot analysis of whole cell extracts to examine the expression of Hif1 truncation mutants in hif1Δ hat2Δ double knockout cells.