Figure 5.

brwl mutants affect mitotis and meiosis. (A-C) brwl^KG0/brwl^MI0 mutants show decrease in the number of mitotically active cells, as stained by a phospho-histone3 S10 (PH3) antibody (red) and counterstained by phalloidin (green), in 10 day old ovaries. This pattern appears abrupt and random by 18^th day where mitotic cells are seen randomly situated in the ovariole. N = 5-7, n = 20 = 25. (D-F) brwl^KG0 /brwl^MI0 mutants show decrease in the number of mitotically active cells, as stained by the Cyclin E antibody (red) in 10 day old ovaries. This pattern is further disrupted by 18^th day (C) where randomly scattered mitotic cells are seen in the ovariole. N = 5, n = 15-35. (G-I) brwl^MI0/brwl^MI0 mutants show complete loss of the formation of synaptonemal complex, as marked by the anti-C(3)G antibody, on the 10^th day post eclosion. 100% of ovarioles show this phenotype. N = 5, n = 15-25.