Figure 6.

Ovarian defects due to loss of brwl are ameliorated by over-expression of stwl. (A-C) brwl^MI0/brwl^MI0 mutants show ovary defects, as measured by DAPI staining (blue) and phalloidin (green) that are rescued in brwl^MI0/ brwl^MI0; nos-Gal4/UAS-stwl-HA on the 10^th (B) and 18^th (C) day PE. The rescue is however not as strong on the 18^th day PE. (D) Quantitative data representing the number of ovarioles showing the phenotype on 3^rd (n = 10), 10^th (n = 56) and 18^th (n = 25) day after fly eclosion. Ovaries from three day old brwl^MIO/brwl^KGO animals were used as a control for statistical analyses. (E-G) brwl^MI0/brwl^MI0; nosGal4/UAS-stwl-HA ovarioles showing reduced oocyte specification defects. Oocyte defects are significantly rescued in brwl^MI0/ brwl^MI0; nosGal4/ UAS-stwl-HA ovarioles on the 10^th day (n = 25). Mild rescue is seen on the 18^th day (n = 15) with a decrease in ovarioles without orb staining. (H) Quantitative data representing the percentage of ovarioles that show the phenotype on third, tenth and eighteenth day after fly eclosion. Ovaries from three day old brwl^MIO/brwl^KGO animals were used as a control for statistical analyses. N = 3-15; n = 25-60. (I-K) brwl^MI0/ brwl^MI0 mutants show apoptotic egg chambers which is not rescued in 10 day old brwl^MI0/ brwl^MI0; nanosGal4/ UAS-stwl-HA ovarioles. (L) Quantitative data representing the percentage of ovarioles that show the phenotype on third, tenth (n = 20) and eighteenth (n = 15) day after fly eclosion. Ovaries from three day old brwl^MIO/brwl^KGO animals were used as a control for statistical analyses. N = 3-10; n = 15-20.