Table 1. Primer sequences of the markers used in this study.

Markers	Forward primer (5′-3′)	Reverse primer (5′-3′)	PCR (bp)	Tm (°C)
Traes_2AS_3381A39C6	GTTCCACACCCAAGATGGTTAC	TGAAAGAAACACTAAGCTGAGGAG	503	55
SNP19777336	TGAATATATAGCAGTATGGGTGGCAG	GTACCAAACGCCATGAAAGGG	1075	55
IWB58709	GGTTGAGTTTCCGAGTTTCGTG	GTTCTTGTTCTCCCGTTTGTGC	1505	58
GWM636-M	TGCGGTAGTTTTTAGCAAAGCG	TTTGTCTGGATAATACGACCCCC	738	56
IWB64569	AGCCCTTGCGGAATTCATGGCAAA	GCTACAGCAGTATGTACACAAAAGCCTG	1351	55
Traes_2AS_5CAF7A367	TCCATCCACAACACCTACCC	GCAGCGTAATAATACTTCCTATGAT	1725	55
IWB72490	CTCCACCGCTCATCTTCCTG	TCCCCCAACCGACATTATCC	1559	55
WMC407-M	GCATCTTGTGTTGGTGTTGCC	TCAAGGGTGCGTTTTTCACTTC	423	53
Traes_2AS_D11AD107B	GTTCGCATCTCCGCCTTTAGG	TGGTCACAGGGTAGAAGTATTTCGG	1617	55
Traes_2AS_27D2CB02A	CAAGACACACAGACCTGCTTTTT	CACTGTAATAAAGTGGTAAAGTGAAGC	1675	53
IWB11136	GTCTACATGAAAATAAGCTGCTGAGG	GATGGAGTTTTGGGTTTAAGTTCTAG	365	52
SNP30671452	TGGAGGAAGACGATGGAGACTG	GCAAACCACCAAAGAAGAACCG	581	61
IWB47995	GCTCCCCAAGGACAACATCA	AGAGAGCCAAGAGCAAGCATACC	497	60