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. 2018 May 25;6:181. doi: 10.3389/fchem.2018.00181

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Copyright © 2018 Das, Pukala and Smid.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) IM-MS spectrum of αSA53T (50 μM) in 50 mM ammonium acetate. (B) Plot of CCS vs. charge state for the dominant peaks in the IM-MS spectrum measured for αSA53T at 0 h (circles) and following incubation in the absence (squares) and presence of 1:2 (protein: compound) honokiol (triangles) or 2-D08 (diamonds). Where multiple features were observed in the ATD, CCS is plotted for each feature. (C) Experimentally derived CCSs for the +11-charge state (m/z 1,317) of αSA53T following 48 h incubation at 37°C with shaking at 300 rpm.