. Author manuscript; available in PMC: 2018 Jun 1.

Published in final edited form as: Hum Mutat. 2017 Dec 26;39(3):415–432. doi: 10.1002/humu.23380

Table 4.

PCSA – Monte Carlo results (straight-line parameterization) from Sedimentation Velocity experiment.

Species:	Molar mass (kDa)	D (x 10⁻⁷ cm/sec²)	s (x 10⁻¹³ sec)	f/f₀	RMSD
WT	118.5 (111.6, 125.5)	4.54 (4.43, 4.66)	5.67 (5.48, 5.85)	1.44 (1.43, 1.45)	0.0025
p.Val155Gly	122.0 (110.6, 133.5)	4.43 (4.20, 4.65)	5.68 (5.42, 5.94)	1.46 (1.44, 1.49)	0.0032
p.Tyr330Cys	120.6 (116.3, 124.9)	4.52 (4.50, 4.55)	5.74 (5.51, 5.97)	1.44 (1.42, 1.46)	0.0028
p.Ser356Asn	120.0 (110.3, 129.6)	4.41 (4.34, 4.49)	5.57 (5.21, 5.93)	1.48 (1.46, 1.49)	0.0034

Values in parenthesis are 95% confidence intervals from the Monte Carlo analysis. The theoretical molar mass of HARS WT dimer is 114.9 kDa, indicating the observed species is a dimer. The residual mean square deviation (RMSD) of the fit is shown in units of absorbance at 230 nm.