REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies or Fluorophores
Phospho-Myosin Light Chain 2 (Ser19)	Cell Signaling Technology	#3671S; RRID: AB_330428
Cleaved Caspase-3 (Asp175) (D3E9)	Cell Signaling Technology	#9579S; RRID: AB_10897512
Goat anti-Rabbit IgG, Alexa Fluor Plus 488	ThermoFisher	Cat. No. A32731; RRID: AB_2633280
BODIPY 558/568 Phalloidin	ThermoFisher	Cat. No. B3475
DAPI	ThermoFisher	Cat. No. D3571
Calcein Green	ThermoFisher	C3100MP
Bacterial and Virus Strains
eGFP Adenovirus	Vector Biolabs	Cat. No. 1060
CFP Adenovirus	Vector Biolabs	Cat. No. 1766
LifeAct GFP Adenovirus	Ibidi	Cat. No. 60121
LifeAct RFP Adenovirus	Ibidi	Cat. No. 60122
Lentivirus Raf1-(RBD)-GFP	Devreotes Lab, AddGene
Lentivirus PH-Akt-GFP	Devreotes Lab, AddGene	#51465
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
LY-294002	Sigma	L9908
CK-666	Sigma	SML0006
Jasplakinolide	Tocris	Cat. No. 2792
Latrunculin A	Tocris	Cat. No. 3973
Y-27632	Calbiochem	Cat. No. 688000
FGF2	Sigma	F0291
Insulin	Sigma	I9278
Gentamicin	ThermoFisher	15750
Trypsin	ThermoFisher	27250-018
Collagenase	Sigma	C2139
FBS	Sigma	F0926
DMEM/F-12	ThermoFisher	10565-018
D-PBS	ThermoFisher	14040-133
Penicillin-Streptomycin	Sigma	P4333
ITS	ThermoFisher	51500-056
Matrigel, Growth Factor Reduced, ~9 mg/mL	Corning	354230
Collagen-I, Rat Tail	Corning	354236
DNase	Sigma	D4263
Critical Commercial Assays
Experimental Models: Organisms/Strains
Mouse: FVB/NJ	The Jackson Laboratory	Stock No: 001800; RRID: IMSR_JAX:001800
Mouse:FVB, Gt(ROSA26)ACTB-tdTomato-EGFP	Andrew Ewald Laboratory	Stock No: 007576; RRID: IMSR_JAX:007576
Mouse: FVB, Tg(KRT14-EGFP/Actb)50Efu; Gt(ROSA26)ACTB-tdTomato-EGFP	Andrew Ewald Laboratory	MGI: 4421514
Software and Algorithms
CellFIT-3D
Imaris, version 8.4		www.imaris.com
Fiji, version 2.0.0-rc-43/1.51j		https://fiji.sc/
Prism 7		https://www.graphpad.com/scientific-software/prism/
Matlab, R2017a		https://www.mathworks.com/
Micromanager, version 1.4		https://micro-manager.org/
Other
ViroMag	OZ Bioscience	#RL400200
24-well glass bottom plates	Greiner Bio-One	662892
96-well low adherent plates	ThermoFisher	174907
Immersion medium Immersol W 2010	Zeiss	444969