Figure 7. PF‐431396 does not inhibit EFS‐evoked Ca²⁺ influx into gastric fundus smooth muscle cells.

A and B, representative images of the Cal‐520 fluorescence evoked by 10 Hz, 10 s EFS of gastric fundus smooth muscles in the presence of 100 μm l‐NAME and 1 μm MRS2500, in the absence (A) or presence (B) of 0.3 μm PF‐431396 (scale bar: 200 μm). C and D, representative traces of the fluorescence intensity values evoked by 10 Hz, 10 s EFS of fundus smooth muscles in the absence (C) or presence (D) of 0.3 μm PF‐431396. E, the mean ± SD of the area under the curve (AUC) of the Ca²⁺ intensity units measured from fundus muscle tissues during 10 Hz, 10 s EFS in the absence or presence of 0.3 μm PF‐431396 (n = 5).