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. 2018 Apr 17;12(6):788–798. doi: 10.1002/1878-0261.12172

Figure 1.

Figure 1

Noxa contributes to cisplatin‐induced apoptosis in a p53‐independent manner. (A) p53‐inactive HN8 and HN12 HNSCC cells were infected with lentiviruses encoding shRNA for nontargeting control or Noxa (shNoxa2). Cells were treated with cisplatin (50 μm for HN8 or 25 μm for HN12) with the indicated periods and equal amounts of the total extracts were used for immunoblot analysis with the indicated antibodies. (B) The cells in (A) were treated with cisplatin for 24 h and cell death was determined by Annexin V‐propidium iodide staining followed by FACS analyses. Another shNoxa construct, shNoxa4 was also introduced in each cell line, which was assayed similarly as shNoxa2. Values represent the mean ± SD of triplicates. The results are representative of at least two independent experiments and are reproducible.