Figure 5.

ATF3 and ATF4 bind to and activate the Noxa promoter through the CRE. (A) HN8 cells were treated with 50 μm of cisplatin for 8 h. Chromatin immunoprecipitation was performed with the indicated antibodies and DNA was analyzed by PCR using primers corresponding to −67 to +60 on the Noxa promoter. (B) HN12 cells were cotransfected with the −131 luc reporter construct and an ATF3 or ATF4 expression plasmid for 24 h. On the next day, cells were treated with 25 μm of cisplatin for 16 h. Values represent the mean ± SD of triplicates. The results are representative of at least two independent experiments and are reproducible.