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. 2018 Apr 18;37(11):e97631. doi: 10.15252/embj.201797631

Figure 1. Hfq is required for correct processing and folding of 16S rRNA .

Figure 1

  1. Schematic representation of the RNase‐mediated processing of the 17S rRNA precursor into mature 16S rRNA.
  2. Northern blot analysis of total RNA extracted from cells in exponential (EXP) or stationary (STAT) growth phase. Samples were fractionated on a 4% polyacrylamide/7 M urea gel. A scheme of the probes binding to the rRNA sequence is displayed on the side.
  3. Electrophoretic mobility shift assays of Hfq binding to the 5′ and 3′ extremities of the 17S rRNA. Increasing amounts of Hfq hexamer were mixed with a constant amount of the specific 17S‐flanking sequences and resolved on a 6% (top panel) or 8% (bottom panel) native polyacrylamide gel.
  4. DMS and CMCT accessibility probing of the 16S rRNA. Reverse‐transcribed cDNA was fractionated on an 10% polyacrylamide/7 M urea gel. Residues with altered reactivities in the Δhfq mutant are indicated. The inset depicts the analyzed region of the 16S rRNA.

Source data are available online for this figure.