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. 2018 Apr 11;596(11):2077–2090. doi: 10.1113/JP275502

Figure 2. HSL redistributes to LDs after exercise as visualised through confocal immunofluorescence microscopy.

Figure 2

A, representative images of HSL and LDs pre and post exercise were initially obtained using a 40× oil objective on a confocal microscope. Scale bars represent 50 μm. LDs were visualised using BODIPY 493/503 and represent IMTG stores within the cell. Muscle fibres that had the highest lipid droplet content were selected for analysis. B, a 16× digital magnification was then applied to the centre of these muscle fibres and images were obtained to identify and quantify distribution and colocalisation between HSL and LDs. Scale bars represent 2 μm. Panel B shows that in the pre‐exercise state HSL appeared as large storage clusters throughout the cell, whereas post‐exercise HSL was visualised as a greater number of smaller, discrete clusters centred on LDs, as observed on the merged images. [Color figure can be viewed at http://wileyonlinelibrary.com]