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. 2018 Apr 11;596(11):2077–2090. doi: 10.1113/JP275502

Figure 5. Immunofluorescence microscopy images of HSL, PLIN2/PLIN5 and LDs before and after exercise.

Figure 5

Colocalisation between HSL, LDs and PLIN2 (A) or PLIN5 (B) as visualised through immunofluorescence microscopy and quantified before and after exercise. Images captured with a 63× oil objective on a confocal microscope at 16× digital magnification in the central region of fibres that had the highest lipid droplet content. Scale bars represent 2 μm. PLIN2 (A) and PLIN5 (B) were associated with LDs or located in the cytosol. HSL redistributes from larger storage clusters to a greater number of smaller, discrete clusters centred on different subclasses of LDs (i.e. PLIN+ or PLIN− LDs). [Color figure can be viewed at http://wileyonlinelibrary.com]