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. 2018 Apr 18;37(11):e97980. doi: 10.15252/embj.201797980

Figure 2. BCR‐independent Igβ expression determines the fitness of Ramos cells.

Figure 2

  • A
    A schematic diagram showing the route map for generating single‐ and multi‐ BCR component KOs from WT Ramos B cells by the CRISPR/Cas9 method.
  • B
    The expression of Igβ on the surface of different BCR component KO Ramos cells was determined by flow cytometry.
  • C
    Growth competition of the BCR component KO cells against gWT cells. The data represent the mean and standard error of a minimum of three independent experiments.
  • D–G
    Calcium responses of WT and BCR component KO Ramos cells upon the stimulation with anti‐Igβ antibodies. HL‐KO = HC/LC double KO; HLα KO = HC/LC, Igα triple KO; HLβ KO = HC/LC, Igβ triple KO. One clone is used for each genotype.