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. 2018 May 15;37(11):e97499. doi: 10.15252/embj.201797499

Figure EV3. pCUC2::3xVENUS‐N7 expression is expanded in the epidermal cells of sup mutants.

Figure EV3

  1. pCUC2::3xVENUS‐N7 in WT floral buds at stages 3‐6. CUC2 was highly expressed in the floral buds of stage 3, and its expression started to be constrained to the boundary regions between the sepals after stage 3. At stage 5 and stage 6 floral buds, CUC2 was only detected in the small boundary regions at the bottom of the sepals.
  2. pCUC2::3xVENUS‐N7 in sup‐5 floral buds at stages 3–6. At stage 3, the CUC2 expression pattern was similar to that in WT. From stage 4, the CUC2 expression pattern in sup‐5 started to show differences from that of WT. CUC2 expression was detected in FM region of at stage 4. At stages 5 and 6, the CUC2 expression was still relatively high but was more concentrated at the bottom of the developing sepals as well as in the broad FM regions. Dashed lines mark the regions with CUC2‐3xVENUS‐N7 in the flower center. “b” indicates the boundary region between sepals; “fm” indicates the floral meristem region in the center.
  3. A cross section of a stage 4 WT floral bud showing a ring of CUC2‐3xVENUS‐N7 signal at the whorl 3/4 boundary.
  4. A cross section of a stage 4 sup‐5 floral bud showing the additional signal of CUC2‐3xVENUS‐N7 in the FM center.
Data information: Scale bars, 20 μm.