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. 2018 Apr 12;37(11):e97780. doi: 10.15252/embj.201797780

Figure 5. YAP overcomes Ras‐induced senescent phenotypes.

Figure 5

  1. Western blot analysis of WI38 cells expressing the indicated retroviral transgenes (here and throughout the figure, 6 days). GAPDH serves as loading control. Two independent experiments.
  2. SAHF (senescence‐associated heterochromatin foci) formation WI38 cells expressing the indicated retroviral transgenes. See Fig EV4A for further controls on Ras. Representative results of a single experiment (> 100 cells per condition); n = 3.
  3. Representative pictures of DAPI staining to visualize SAHF quantified in (B). Scale bar: 15 μm.
  4. Induction of SAβgal (senescence‐associated β‐galactosidase) activity in WI38 cells. Representative results of a single experiment (> 100 cells per condition); n = 3.
  5. Representative pictures of SAβgal staining quantified in (D). Scale bar: 20 μm.
  6. qPCR for SASP (senescence‐associated secretory phenotype) marker genes in WI38 cells. n = 6.
  7. Quantification of proliferating WI38 cells as measured by EdU incorporation. Control and RRM2 siRNAs were transfected 1 day after starting selection. Representative results of a single experiment (> 100 cells per condition); n = 4.
  8. WI38 cells were processed as in (G) but stained for SAHF. Representative results of a single experiment (> 100 cells per condition); n = 3.
Data information: Data are mean and SD with *P < 0.05 (two‐tailed Student's t‐test).