Table 7.

Characteristics of the primers and the real-time qPCR conditions.

	Primer Sequences (5′-3′) 2	Accession Number	Size (Base) 3	Tm (°C) 4	Tissue 5	Linear Dynamic Range(ng RNA) 6	E 7
SLC2A1 1	F-AGATGATGCGGGAGAAGAAG	BC119940	238	89	L	6.25–100	1.86 (0.98)
	R-TCCACCACAAATAGCGACAC				M	6.25–100	1.99 (0.95)
SLC2A4	F-CCAACTGGACATGCAACTTCATC	BC114082	206	85	M	3.13–67.5	1.90 (0.99)
	R-CACTTCCTGCTCCAGAAGAGA
FBP1	F-CAACATCGACTGCCTTGTGT	BC102974	224	88	L	1.56–50	1.97 (0.99)
	R-CCCTGTCCACCAAAATGAAC
PCK1	F-AACTCGCTCCTTGGGAAGAA	BC112664	261	89	L	3.13–50	1.91 (0.99)
	R-CCGCAAGTTACCTTGTTGGT
PCK2	F-CATTGACGCCATCATCTTTG	BC102244	150	88	L	3.13–50	1.87 (0.99)
	R-TGCATGATGACCTTCCCTTT
PC	F-CTATGTTGCCCACAGCTTCA	BC114135	244	90	L	3.13–50	1.93 (0.98)
	R-GATGTCCATGCCATTCTCCT
CPT1A	F-CCTATTTTGGACACGGGAAA	XM_002699420.1	172	86.5	L	3.13–50	1.78 (0.99)
	R-TCAAACCACCTGTCGAAACA
HMGS2	F-GGCCTTTCACTCTCGATGAT	BC112666	170	85	L	1.56–50	1.93 (0.99)
	R-TTCCAGCTTTAGTCCCCTGA
HMGL	F-CAGCTTCGTGTCTCCCAAAT	BC118276	227	87	L	3.13–50	1.87 (0.99)
	R-ACCGCTGCAAACTCTCATCT
BDH2	F-TATCCGGTGCAACTGTGTGT	BC102567	204	84	L	3.13–50	2.01 (0.98)
	R-ACAGGATTCCCCGTGATGTA
ACCA	F-ATCCGACGCCTTACTTTCCT	AJ132890	193	82.5	L	3.13–50	2.05 (0.98)
	R-TTCTCATCCGGTTCAGCTCT
FASN	F-AACACCTCGGTGCAGTTCAT	AY343889	196	88	L	3.13–50	1.94 (0.99)
	R-CTTTCTCTTTGCAGCCATCC
GPAM	F-GCCAGATGGACGGAAAGATA	AY515690	182	83.5	L	3.13–50	2.00 (0.97)
	R-TCGTATTGGTGGACAGCGTA
DGAT1	F-CTCTGTGCCTGGTCATTGTG	BC118146	239	90	L	3.13–50	1.89 (0.99)
	R-CCGGTAGGAGAACAGCTTGA
ACSL1	F-GAACTACAGGCAACCCCAAA	BC119914	240	83	L	3.13–50	1.94 (0.99)
	R-GGGCCTTGAGATCATCCATA
GUSB	F-ACCAGAAGCCGATGATTCAG	BC133415	160	84	L	3.13–75	1.96 (0.99)
	R-AGCTCGCCAACCACATATTC				M	6.25–100	1.76 (0.96)

SLC2A1: glucose transporter 1, FBP1: fructose-1,6-bisphosphatase 1, PCK1 and PCK2: phosphoenolpyruvate carboxykinase 1 and 2, PC: pyruvate carboxylase, CPT1A: carnitine palmitoyltransferase 1A, ACSL1: acyl-CoA synthetase long-chain family member 1, HMGS2: 3-hydroxy-3-methylglutaryl-CoA synthase 2, HMGL: 3-hydroxymethyl-3-methylglutaryl-CoA lyase, BDH2: 3-hydroxybutyrate dehydrogenase, type 2, ACCA: acetyl-CoA carboxylase, FASN: fatty acid synthase, GPAM: mitochondrial glycerol phosphate acyltransferase, DGAT1: diacylglycerol O-acyltransferase homolog 1, GUSB: β-glucuronidase. ¹ Primer concentration and annealing temperature for this gene was 1.0 µM and 58 °C; ² F-forward, R-reverse, Exon-exon junctions are underlined; ³ Amplicon sizes of PCR products; ⁴ Melting temperatures of PCR products; ⁵ Tissues which were measured for the gene. L: liver, M: muscle; ⁶ The range of RNA input (ng) in which linearity of standard curve was confirmed; ⁷ Amplification efficiency (E) and r² in parenthesis determined by dilution method.