Table 1.
Name of Isolate | Identified with vip1 Primers | Identified with vip2 Primers | ||||
---|---|---|---|---|---|---|
Similarity (%) ‡ | Coverage (%) * | Closest Homolog | Similarity (%) ‡ | Coverage (%) * | Closest Homolog | |
V-J20.2 | 100 | 44 | vip1Bb1 | 97 | 70 | vip2Bb1 |
V-LE1.1 | 100 | 40 | vip1Bb1 | 99 | 72 | vip2Bb1 |
V-V54.26 | 99 | 49 | vip1Ca1 | 99 | 71 | vip2Ac1 |
V-V54.31 | 100 | 49 | vip1Ca1 | 98 | 73 | vip2Ac1 |
E-SE10.2 | 62 | 30 | vip1Bb3 | No DNA amplification | ||
E-TE7.43 | 100 | 49 | vip1Ca1 | 99 | 64 | vip2Ac1 |
E-TE16.5 | 98 | 43 | vip1Ca1 | 98 | 73 | vip2Ac1 |
E-TE18.40 | 100 | 30 | vip1Ca1 | 100 | 45 | vip2Ac1 |
O-V84.2 | 40 | 40 | vip4Aa1 | No DNA amplification |
* The coverage values represent the mean of two replicates of the typing or screening PCR products to the full sequence length of the respective vip1, vip2 and vip4 genes deposited in GenBank. ‡ The similarity values provided by BlastX (NCBI) represent the mean value of the in silico translation of two replicates of the PCR products.