Table 4.
Identification of expressed proteins from the identified putative insecticidal protein genes in the concentrated supernatant and in the solubilized proteins from the spore/crystal mixture of the Bt isolates E-SE10.2 and O-V84.2 by in gel digestion LC/MSMS analysis *.
| Sample | Protein Identity | Mass Protein (kDa) | SignalIP Server 4.1 | Supernatant (LB) | Spore/Crystal Mixture (CCY) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | ||||||||||
| Rep. 1 | Rep. 2 | Rep. 3 | Rep. 1 | Rep. 2 | Rep. 3 | Rep. 1 | Rep. 2 | Rep. 3 | ||||
| E-SE10.2 | Vip2Ac-like_1 | 51.6 | Yes | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | −/− | −/− | −/− |
| Sip1Aa-like_1 | 40.7 | Yes | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | −/− | −/− | −/− | |
| Cry23Aa-like | 29.3 | No | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | |
| O-V84.2 | Vip4Aa-like_1 | 97.5 | Yes | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | −/− | −/− | −/− |
| Vip2Ac-like_2 | 80.79 | Yes | +/+ | +/+ | +/+ | −/+ | +/+ | +/+ | −/− | −/− | −/− | |
| Vip4Aa-like_2 | 87.5 | Yes | +/+ | +/+ | −/+ | −/− | −/− | −/− | −/− | −/− | −/− | |
| Vip2Ac-like_3 | 23.2 | Yes | −/− | −/+ | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |
| Sip1Aa-like_2 | 38.7 | Yes | −/− | −/+ | +/+ | −/− | −/− | −/− | −/− | −/− | −/− | |
| Cry45Aa-like_1 | 30.6 | No | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ | |
| Cry45Aa-like_2 | 29.3 | No | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ | |
| Cry45Aa-like_3 | 25.6 | No | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ | |
| Cry32Ea-like | 151.2 | No | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ | |
| Cry32Da-like | 153.7 | No | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ | |
| Cry32Eb-like | 76.8 | No | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ | |
| Cry73Aa-like | 72.2 | No | −/− | −/− | −/− | −/− | −/− | −/− | +/+ | +/+ | +/+ | |
* +/+, the insecticidal protein genes were identified with Protein Pilot (Paragon algorithm) and Mascot; +/−, the insecticidal protein genes were identified with Protein Pilot (Paragon algorithm) but not with Mascot; −/+, the insecticidal protein genes were identified with Mascot but not Protein Pilot (Paragon algorithm); −/−, the insecticidal protein genes were not identified with either Protein Pilot (Paragon algorithm) or Mascot.