. 2018 May 10;10(5):193. doi: 10.3390/toxins10050193

Table 5.

Estimation of the relative production expressed as weight percentage of the insecticidal protein genes in the supernatant and solubilized proteins from the spore and crystal mixtures of the Bt isolates E-SE10.2 and O-V84.2 identified with Mascot.

Supernatant	24 h (% Weight)			48 h (% Weight)
Supernatant	R1	R2	R3	R1	R2	R3
E-SE10.2
Non Secretable toxins	98.56	99.06	99.36	97.87	99.26	99.63
Flagellin protein FlaA	55.21	73.82	87.19	39.75	36.04	43.19
Secretable toxins	1.44	0.94	0.64	2.13	0.74	0.37
Vip2Ac-like_1	0.09	0.06	0.03	0.09	0.10	0.06
Sip1A-like_1	0.09	0.03	0.02	0.06	0.08	0.02
Cry23Aa-like *	1.26	0.85	0.59	1.98	0.56	0.29
O-V84.2
Non Secretable toxins	99.99	99.99	99.99	99.99	99.99	99.99
Flagellin protein FlaA	99.20	99.37	98.35	84.14	99.15	88.35
Secretable toxins	0.0032	0.0012	0.0030	0.0111	0.00007	0.0075
Vip4Aa-like_1	0.0014	0.0008	0.0016	0.0019	0.00004	0.0029
Vip4Aa-like_2	0.0005	0.0001	0.0002	-	-	-
Vip2Ac-like_2	0.0013	0.0002	0.0010	0.0092	0.00003	0.0046
Crystal	72 h (% Weight)
Crystal	R1		R2		R3
E-SE10.2
Non-crystal toxins	69.52		95.26		97.51
Crystal toxins	30.48		4.74		2.49
Cry23A-like	30.48		4.74		2.49
O-V84.2
Non-crystal toxins	52.86		51.12		53.25
Crystal toxins	47.14		48.88		46.75
Cry45Aa-like_1	2.82		2.46		1.41
Cry45Aa-like_2	3.05		2.03		3.38
Cry45Aa-like_3	1.88		5.29		2.20
Cry32Ea-like	24.30		25.06		25.89
Cry32Da-like	6.10		5.40		4.60
Cry32Eb-like	6.20		4.46		5.02
Cry73Aa-like	2.79		4.18		4.25

* The Cry23Aa-like protein was detected in the supernatant and the crystal of the Bt isolate E-SE10.2, but, according to the prediction of the SignalIP server 4.1, it is most likely not secretable.