Table 1.
Spa-types and in vitro LukMF’ production of field isolates from bovine clinical and subclinical mastitis cases.
| Spa-Type | Isolates N | Clinical Mastitis N (%) | lukM-lukF’ Postive Isolates N (%) 1 | High LukMF’ Production Isolates N (%) 2 | Clonal Complex 3 |
|---|---|---|---|---|---|
| t529 | 40 | 23 (58) | 40 (100) | 0 (0) | CC151 |
| t543 | 12 | 12 (100) | 12 (100) | 12 (100) | CC479 |
| t524 | 1 | 1 (100) | 0 (0) | 0 (0) | ND 4 |
| t1403 | 1 | 1 (100) | 1 (100) | 0 (0) | CC133 |
| t015 | 1 | 0 (0) | 0 (0) | 0 (0) | ND 4 |
| Total | 55 | 37 (67) | 53 (96) | 12 (22) |
1 Number of lukM-lukF’ positive isolates. Genes detected by PCR. 2 Number of samples with >10 µg/mL LukMF’ production after eight hours culture, measured in supernatant by ELISA. 3 Clonal complex determined by MLST based on whole genome sequences of subset of isolates from this spa-type. 4 Not determined.