Table 2.

Primers and annealing temperature used in this study.

Gene	Sequence	Product Size (bp)	Annealing Temperature (°C)	Reference
femA	f: 5′-tgcctttacagatagcatgcca-3′	142	59.5	[40]
	r: 5′-agtaagtaagcaagctgcaatgacc-3′
lukM	f: 5′-aaacgcgcagttaataaaaag-3′	975	55	This study
	r: 5′-agcattaggtcctcttgtcg-3′
lukF’	f: 5′-actcaggctatacccaaccca-3′	472	59.5	This study
	r: 5′-cgagctactctgtctgccac-3′
rot	f: 5′-accaatttagcctcattcggtttg-3′	705	55	This study
	r: 5′-catcgtcaacaggacgctct-3′