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. 2018 May 11;16(5):158. doi: 10.3390/md16050158

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression analysis of recombinant SAGL in fermentor. (a) SDS-PAGE analysis of SAGL expression in fermentor. BSM medium was used in test. Culture supernatant samples collected at 12 h intervals were tested by electrophoresis. The target protein bands represented rSAGL were marked by arrows; (b) Western blot of culture supernatant of recombinant yeast. Lane M protein marker; culture samples from fermenters at 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, and 144 h were tested in turn at the following lanes.