Table 1.

Primers used in this experiment

LI:	TCC CCCGGG ATG ATG ATG GCG TCT AAG GAC	L2:	GC TCTAGA TTA TWW WCG GCG CAV WCC AAG CC
G1:	TCC CCCGGG CTT GCA ATG GAT CCT GTG GC	Ql:	GC TCTAGA ACT GCT GTT GAA GAA CCC GC
G2:	TCC CCCGGG CAA GGT GAA TTT ACT ATT TC	Q2:	GC TCTAGA AAT AGT AAA TTC ACC TTG GG
G3:	TCC CCCGGG CTT AAT CCC TTC TTG TTA C	Q3:	GC TCTAGA TGC CAA CCC AGC CAT TAT AC
G4:	TCC CCCGGG GGT AAA ATT ATA GTT TCT TGC	Q4:	GC TCTAGA AAT CAC ATG CGG GAA GAG AG
G5:	TCC CCCGGG GAT GTA AGG AAT GTT CTC TTT C	Q5:	GC TCTAGA CTT ACA TCT ACT AAG GGT AC
G6:	TCC CCCGGG CAA ACA ATG CGC CTT GTG TGC	Q6:	GC TCTAGA GGA GGG GGG TGT AAA GCA TG
G7:	TCC CCCGGG ACT TGT CCT AGC CCC GAT TTC	Q7:	GC TCTAGA GTT CCA CTG TGG GAG GAA CC
G8:	TCC CCCGGG TTG TCA AAT TCA CGT GCC CC	Q8:	GC TCTAGA AAT TGG AAG AGG GGC ACG TG
G9:	TCC CCCGGG TGT ACC TTA GAT GGA CGA C	Q9:	GC TCTAGA GGT GGT ACC AAC AAG TCG TC
G10:	TCC CCCGGG GGT ACT TCT AAT GGT ACT GTG	Q10:	GC TCTAGA GGG GCA GGG CCT TCA AAA GG
G11:	TCC CCCGGG GAT TGG CAT ATT AAC ATG AC	Q11:	GC TCTAGA TGA GTC TGG CTA GAA TGT CC
G12:	TCC CCCGGG GGT TCA ATC CAG GCG AAT GG	Q12:	GC TCTAGA TAA GAA CAC CAA TGT AGT TG
G13:	TCC CCCGGG AAG ATC CCC AAC TAT GGG TC	Q13:	GC TCTAGA CTG TGA TGC TAG ACC CAT AG
G14:	TCC CCCGGG TTC ATG TCA AAG ATA CCA GG	Q14:	GC TCTAGA AAG CAC CAG GGC CTG GTA TC
G15:	TCC CCCGGG CCC ACT GTT GGT GAA GCC GC	Q15:	GC TCTAGA TAG TGG AGT AGG GCG GCT TC
G16:	TCC CCCGGG GAT GGT TTC CTA ACC TGT GTC	Q16:	GC TCTAGA GCT GGC CCC GTT AGG GAC AC
Trx-up: TTC CTC GAC GCT AAC CTG GC		Trx-DW: GTA AA CGA CGG CCA GTG CC

G, L and Q primers were used for amplifying NV96-908 fragments, and Trx-up and Trx-dw primers for generating the insert and junction sequence of recombinant plasmids. These Trx primers have also been used for sequencing. Underlined sequences of G primers and LI primer shows the SmaI site, and that of bo th L2 primer and Q primers the XbaI site.