Table 2.
Characterization and reactivity of MAbs to whole rNV96-908, and its N-terminal and C-terminal fragments(rNV-N, rNV-C).
| Hybridoma | Isotype | Reactivity in ELISA | Reactivity in WB | ||||
| (type of light chains) | whole rNV96-908 | rNV-N | rNV-C | whole rNV96-908 | rNV-N | rNV-C | |
| 3E7 | IgG1 (k) | ++ | ++ | - | ++ | ++ | - |
| 4E6 | lgG2b (k) | ++ | ++ | - | ++ | ++ | - |
| 4F7 | lgG2a (k) | ++ | ++ | - | ++ | ++ | - |
| 7D2 | lgG2b (k) | ++ | ++ | - | ++ | + | - |
| 7E9 | lgG2b (k) | ++ | ++ | - | - | - | - |
| 13F9 | lgG2b (k) | ++ | ++ | - | ++ | ++ | - |
| 1G9 | Not cloned | ++ | ++ | - | ++ | ++ | - |
| 3D10 | Not cloned | ++ | ++ | - | ++ | ++ | - |
| 4B10 | Not cloned | ++ | ++ | - | ++ | ++ | - |
| 5B6 | Not cloned | ++ | - | ++ | ++ | - | ++ |
| 6C4 | Not cloned | ++ | ++ | - | ++ | ++ | - |
| 8C7 | Not cloned | ++ | - | ++ | ++ | - | ++ |
| 8D2 | Not cloned | ++ | ++ | - | ++ | ++ | - |
| 10F8 | Not cloned | ++ | ++ | - | ++ | ++ | - |
| 10G10 | Not cloned | ++ | - | ++ | ++ | - | ++ |
| 12D4 | Not cloned | ++ | ++ | - | ++ | ++ | - |
| 15G5 | Not cloned | ++ | ++ | - | ++ | ++ | - |
- Negative reaction; + positive reaction; ++ strong positive reaction