Figure 2.

Anti-proliferative activity of Aeroplysinin-1 and Isofistularin-3 on pheochromocytoma cells in monolayer culture. (A) Aeroplysinin-1 decreased the viability of all three pheochromocytoma cell lines significantly after 24 h treatment. Isofistularin-3 only affected the viability of the mouse pheochromocytoma cells, whereas, the PC12 rat pheochromocytoma cells was not affected up to a concentration of 100 µM under normoxic and hypoxic conditions. Cultivation under hypoxia increased the necessary effective concentration to reduce the viability to 50% (EC₅₀). (B) Furthermore, the effect on the number of proliferating cells was analyzed under normoxic and hypoxic conditions. Treatment with 1 µM Aeroplysinin-1 reduced the number of proliferating cells in all three cell lines in trend. Under hypoxic conditions (1% oxygen), pheochromocytoma cells stopped cell division. Four to five independent experiments were performed (n = 4–14). Average ± standard error of the mean (SEM); Analysis of variance (ANOVA) and Bonferroni post hoc test comparison vs. control * p < 0.05, ** p < 0.01.