Spheroid growth inhibition by a single or fractionated treatment with Aeroplysinin-1. (A) Spheroids were treated with different concentrations Aeroplysinin-1 after completed spheroid formation (day 4) and growth was monitored over a time period of 14 days. Furthermore, (B) a fractionated treatment on day four, 8, 11, and 15 took place. Three to four independent experiments (n = 15–48). Average ± SEM; ANOVA and Bonferroni post hoc test comparison vs. control * p < 0.05, ** p < 0.01; or vs. 5 µM Aeropylysinin-1 # p < 0.05. Arrows mark the different treatment time points. Scale bar: 200 µm.