Figure 5.

Aeroplysinin-1 influences the pro-metastatic behavior of MTT cells. Impact of different concentrations of Aeroplysinin-1 on (A) MTT cell migration and (B) invasion were analyzed in Boyden-Chamber assays (with (B) or without (A) Matrigel coating) after 24 h. Furthermore, the adhesion capacity of MTT cells to (C) collagen and (D) fibronectin after 24 h treatment with Aeroplysinin-1 was determined. (E) Spheroid formation was tracked over four days and the spheroid diameter was analyzed four days after seeding. Three independent experiments (n = 12–18). Average ± SEM; ANOVA and Bonferroni post hoc test comparison vs. control * p < 0.05 or ** p < 0.01. Scale bar: 200 µm.