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. 2018 May 14;128(6):2600–2612. doi: 10.1172/JCI98709

Figure 4. 2KR mice are protected from DIO.

Figure 4

(A) BW curve of mice that started HFD feeding at 6 weeks old. *P < 0.05 for WT vs. 2KR. n = 16 male (m) WT; n = 15 male 2KR; n = 14 female (f) WT; n = 13 female 2KR. (B) Body composition of male mice after 8 weeks of HFD feeding. *P < 0.05; **P < 0.01 for WT vs. 2KR. n = 10 WT; n = 9 2KR. (C and D) i.p. GTT (C) and AUC (D) in male mice at 12 weeks of HFD feeding. *P < 0.05 for WT vs. 2KR. n = 10 WT; n = 9 2KR. (E) PTT in male mice at 12 weeks of HFD feeding. *P < 0.05; **P < 0.01 for WT vs. 2KR. n = 10 WT; n = 5 2KR. (F and G) Male mice started HFD feeding at 6 weeks old and were sacrificed after 24 weeks on HFD feeding after overnight fasting, followed by 4 to 6 hours of refeeding. (F) Improved obesity-associated hepatic steatosis in 2KR mice indicated by histological analysis of the livers by H&E and oil red O staining. Original magnification, ×100. (G) qPCR analysis of hepatic lipogenic gene expression. *P < 0.05; **P < 0.01 for WT vs. 2KR. n = 7 WT; n = 5 2KR. Data represent mean ± SEM. Student’s t test was used for statistical analyses.