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. 2018 Apr 23;128(6):2239–2251. doi: 10.1172/JCI96764

Figure 2. Design and characterization of functional BiIAs.

Figure 2

(A and B) Schematic diagrams show the structures of BiIA-DG and BiIA-SG bearing the scFv binding domain(s) to gp120 (orange VH/VL of PGT128) and to human CD4 (green VH/VL of Hu5A8). (C) The released BiIA-DG, BiIA-SG, and parental IAs were determined in culture supernatants by Western blot analysis under nonreducing conditions (NR, whole left panel) or reducing conditions (R). (D) ELISA binding profiles of BiIA-DG and BiIA-SG to human sCD4 and HIV-1JR-FL gp120, respectively, compared with IA-PGT128, IA-Hu5A8, and VRC01-IgG. Data represent mean ± SD with duplicates. All experiments were repeated at least twice.