Figure 1. Regulation of Uhrf1 expression in response to mediators contributing to VSMC phenotypic switching.

(A) Analysis of the expression of 96 epigenetic genes in PDGF-BB–treated primary murine VSMCs with microfluidic cards. After global normalization, the statistical analysis was performed on 2 experimental duplicates compared with control cells (grown in a serum-deprived condition) and plotted versus the values of fold changes. A gene was arbitrarily considered modulated if fold induction was <0.6 to >2 compared with control and if the P value of such a difference was less than 0.05. (B) RT-qPCR gene expression analysis of VSMCs treated with different stimuli. The control sample (0.1% FBS) was set as 1. Results are the average of at least 3 independent experiments, and error bars indicate SD. ^#P < 0.05. (C) Representative Western blot showing UHRF1 levels in VSMCs treated with PDGF-BB (25 ng/ml) or TGF-β (10 ng/ml). Unpaired 2-tailed Student’s t test was used to compare means for A, and 1-way ANOVA with Tukey’s multiple comparisons test was used for B. ^#Adjusted P < 0.05.