Figure 4. Effect of Uhrf1 modulation in an in vivo model of restenosis.

(A) Representative H&E staining and quantification of carotid sections of ApoE^–/– mice infused with shSCR or shUHRF1 viruses undergoing collar placement. (B) Representative H&E staining and experimental quantification of carotid sections of Uhrf1 WT (ApoE^–/–UHRF1^wt/wtcre/ER^T2) and KO (ApoE^–/–UHRF1^fl/flcre/ER^T2) mice undergoing collar placement. (C) Representative immunostaining and quantification for Ki67 on carotid sections of Uhrf1 WT and KO mice undergoing collar placement (Ki67 in brown). (D) Representative TUNEL staining and quantification of carotid sections from Uhrf1 WT and KO mice undergoing collar placement (TUNEL⁺ cells in green). Error bars indicate SD. To compare means, we used the Mann Whitney U test in A and B and the unpaired 2-tailed Student’s t test in C and D. Scale bars: 100 μm.