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. 2018 May 7;128(6):2473–2486. doi: 10.1172/JCI96121

Figure 6. Role of Uhrf1 on VSMC function and differentiation.

Figure 6

(A and B) RT-qPCR and Western blot showed an increase in several differentiation markers in Uhrf1-silenced VSMCs (cells cultured in medium with 10% FBS). (C) VSMC differentiation marker expression in Uhrf1-silenced cells treated with PDGF-BB (cells cultured in medium with 0.1% FBS). (D) VSMC differentiation marker expression in cells overexpressing human UHRF1 treated with TGF-β (cells cultured in medium with 0.1% FBS). EV, empty vector; UHV, UHRF1 vector. (E) Schematic illustration representing data shown in A–D. The results are the average of at least 3 independent experiments. Error bars indicate SD. To compare means, unpaired 2-tailed Student’s t test was used for D and 1-way ANOVA with Tukey’s multiple comparisons t test was used for A and C. #P < 0.05 (P value is adjusted only in A and C).