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. 2018 May 14;128(6):2626–2641. doi: 10.1172/JCI99350

Figure 7. Blockade of galanin receptors in the VHT of WT C57BL mice prevents ΔFosB-mediated increase in energy and reduces bone density.

Figure 7

(A) WT C57BL mice in which the VHTs were stereotactically injected with ΔFosB AAV or GFP AAV, and nonselective galanin receptor blocker M35 was delivered for a period of 2 weeks via intrascapularly positioned osmotic pump connected to third ventricle–implanted cannula. (B) Weight gain at 2 weeks after surgery. (C) Calorimetric analysis of energy expenditure. (D) Representative images of eWAT with average weight (grams). (E) Weight gain and percentage of body fat calculated from eWAT weight in relation to body weight. (F) Micro-CT analysis of femurs (see Table 3 for values). (G) Serum P1NP and CTX. Statistical analysis included ANOVA followed by Tukey-Kramer HSD test (n = 5–6), P < 0.05. Groups marked by different letters significantly differ from each other (e.g., a is significantly different from b, but a is not significantly different from ab).